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IMAT Recombinant DNA and PCR questions

42 questions · Biology

Recombinant DNA techniques: restriction enzymes, ligase, vectors and plasmids, and the steps of the polymerase chain reaction (PCR) and what each one does.

Sample questions

  1. 1IMAT Doutor

    To clone a human gene in a bacterium so that a functional protein is produced, it is preferable to start from cDNA rather than genomic DNA because:
    1. AcDNA already carries a bacterial promoter
    2. BcDNA contains no introns, which bacteria cannot remove
    3. CcDNA is more stable than genomic DNA
    4. DcDNA is single-stranded and easier to insert
    5. Egenomic DNA cannot be cut by restriction enzymes

  2. 2IMAT 2026 Q18

    Restriction enzymes are:
    1. AEndonucleases
    2. BIsomerases
    3. CTransferases
    4. DOxidoreductases
    5. ELigases
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